Frequently Asked Question

Questions asked by users might not always be phrased in the clearest way.

Enlist the methods in detect colourless spots in TLC

Accepted Answer

Several methods can be used to detect colorless spots in thin-layer chromatography (TLC). One common method is to use UV light. Many compounds absorb UV light, and when they do, they will appear as dark spots on the TLC plate. Another method is to use a chemical reagent that reacts with the compound to produce a colored product. Finally, some compounds can be detected by their fluorescence. When these compounds are exposed to UV light, they will emit light of a different wavelength, which can be detected by the human eye or a UV lamp.


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