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Enlist the methods in detect colourless spots in TLC (short question)
Accepted Answer
Methods to visualize colorless spots in TLC:
1. Iodine chamber: Iodine vapors react with many organic compounds to form brown complexes.
2. UV lamp: Many organic compounds absorb UV light and fluoresce, allowing for visualization under a UV lamp.
3. Derivatization: React the TLC plate with a reagent that converts the colorless compound into a colored derivative.
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