Frequently Asked Question

Questions asked by users might not always be phrased in the clearest way.

Can you give me a good example of an eluent which might work, when trying column chromatography? I'm trying to separate isobutanol and isobutyl oleate

Accepted Answer

A good eluent for separating isobutanol and isobutyl oleate using column chromatography would be a mixture of hexane and ethyl acetate. Hexane is a non-polar solvent, while ethyl acetate is more polar. By gradually increasing the proportion of ethyl acetate in the eluent, you can exploit the difference in polarity between the two compounds. Isobutanol, being more polar, will elute later than isobutyl oleate.


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