Top topic this week
Back to FAQs
Related FAQs
Frequently Asked Question
Questions asked by users might not always be phrased in the clearest way.
Describe atomic absorption spectroscopy, how substances are detected and quantified
Accepted Answer
Atomic absorption spectroscopy (AAS) is a technique that measures the absorption of light by atoms in a sample. Here's how it works:
1. Sample Preparation: The sample is first dissolved in a suitable solvent and then introduced into the instrument.
2. Atomization: The sample is then passed through a high-temperature flame or a graphite furnace, which breaks down the molecules into individual atoms.
3. Light Source: A beam of light from a hollow cathode lamp, specific to the element being analyzed, is directed through the atomized sample.
4. Absorption: Atoms of the element in the sample absorb light at specific wavelengths, corresponding to their electronic transitions.
5. Detection: The amount of light absorbed is measured by a detector.
6. Quantification: The amount of light absorbed is directly proportional to the concentration of the analyte in the sample. By comparing the absorbance to a calibration curve, the concentration of the element can be determined.
In summary, AAS detects and quantifies substances by measuring the absorption of light by free atoms in a sample. The technique is highly sensitive and can be used to analyze trace amounts of metals and other elements in various matrices.
Articles you might like