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Disadvantage of TLC
Accepted Answer
TLC technique suffers from several disadvantages:
1. Low sensitivity: TLC is less sensitive than other chromatographic techniques, such as HPLC or GC. This means that it may not be possible to detect small amounts of a compound using TLC.
2. Poor resolution: TLC has a lower resolution than other chromatographic techniques, which means that it may not be possible to separate closely related compounds using TLC.
3. Limited sample capacity: TLC plates can only hold a small amount of sample, which limits the amount of compound that can be analyzed.
4. Time-consuming: TLC can be a time-consuming technique, as it requires the preparation of the plate, the application of the sample, the development of the plate, and the visualization of the spots.
5. Limited automation: TLC is not easily automated, which can make it difficult to perform large numbers of analyses.
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