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How will determine the concentration of calcium ion in plasma
Accepted Answer
Procedure:
1. Collect a blood sample in a tube containing an anticoagulant to prevent clotting.
2. Centrifuge the sample to separate the plasma from the red blood cells.
3. Dilute the plasma sample with a known volume of distilled water.
4. Use a calcium ion selective electrode to measure the calcium ion concentration in the diluted sample.
5. Calculate the concentration of calcium ions in the original plasma sample using the dilution factor.
Principle:
The calcium ion selective electrode is a potentiometric sensor that responds to the concentration of calcium ions in a solution. The electrode consists of a glass membrane that contains a calcium-selective ionophore. The ionophore is a molecule that binds specifically to calcium ions. When the electrode is placed in a solution containing calcium ions, the ionophore binds to the calcium ions and forms a complex. The complexation of calcium ions by the ionophore causes a change in the electrical potential across the glass membrane. The change in electrical potential is measured by a voltmeter and is proportional to the concentration of calcium ions in the solution.
1. Collect a blood sample in a tube containing an anticoagulant to prevent clotting.
2. Centrifuge the sample to separate the plasma from the red blood cells.
3. Dilute the plasma sample with a known volume of distilled water.
4. Use a calcium ion selective electrode to measure the calcium ion concentration in the diluted sample.
5. Calculate the concentration of calcium ions in the original plasma sample using the dilution factor.
Principle:
The calcium ion selective electrode is a potentiometric sensor that responds to the concentration of calcium ions in a solution. The electrode consists of a glass membrane that contains a calcium-selective ionophore. The ionophore is a molecule that binds specifically to calcium ions. When the electrode is placed in a solution containing calcium ions, the ionophore binds to the calcium ions and forms a complex. The complexation of calcium ions by the ionophore causes a change in the electrical potential across the glass membrane. The change in electrical potential is measured by a voltmeter and is proportional to the concentration of calcium ions in the solution.
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