Top topic this week
Back to FAQs
Related FAQs
Frequently Asked Question
Questions asked by users might not always be phrased in the clearest way.
If you use dilutions 10-5, 10-6 and 10-7 for spread plate, what dilutions should you use for pour plate?
Accepted Answer
The appropriate dilutions for pour plates would depend on the concentration of the original sample and the desired number of colonies on the plate. Since pour plating involves mixing the diluted sample with molten agar, the final dilution in the plate will be slightly higher than the initial dilution used. Typically, you would use dilutions similar to those used for spread plates, but slightly lower to account for the dilution in the agar. For example, if you used 10-5, 10-6, and 10-7 for spread plates, you could use 10-4, 10-5, and 10-6 for pour plates. However, the optimal dilutions should be determined based on the specific experimental conditions.
Articles you might like