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The Lyman series of lines are used to calculate ionization energy in the hydrogen spectrum because they correspond to the electronic transitions from higher energy levels to the ground state (n = 1) of the hydrogen atom. The ionization energy is the energy required to remove an electron from the atom, and it can be calculated from the wavelength of the emitted light using the equation E = hc/λ, where h is Planck's constant, c is the speed of light, and λ is the wavelength of the light.