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Mobile phase for Squalene in Reverse phase HPLC

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A suitable mobile phase for the analysis of squalene in reverse phase HPLC would be a mixture of water and acetonitrile, with a small amount of organic modifier such as methanol or isopropanol. The exact composition of the mobile phase will depend on the specific column and detection method used. For example, a mobile phase of 70% water, 30% acetonitrile, and 2% methanol would be a good starting point for a C18 column with UV detection.

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