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Prepare 30 ml of a 5 mg/ml oral phenobarbital solution using phenobarbital available from the manufacturer in a 65 mg/ml solution that comes in 1-ml vials. How much stock solution will be required?
Accepted Answer
You will need 9.23 mL of the 65 mg/mL stock solution. Here's how to calculate that:
1. Desired Concentration: 5 mg/mL
2. Desired Volume: 30 mL
3. Stock Concentration: 65 mg/mL
Formula: (Desired Concentration x Desired Volume) / Stock Concentration = Volume of Stock Solution Needed
Calculation: (5 mg/mL x 30 mL) / 65 mg/mL = 9.23 mL
To prepare the solution:
1. Carefully withdraw 9.23 mL of the 65 mg/mL stock solution from a vial using a calibrated syringe or pipette.
2. Transfer the stock solution to a 30 mL volumetric flask or graduated cylinder.
3. Add purified water to the flask or cylinder to reach the 30 mL mark, ensuring thorough mixing.
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