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Propose a successful method to quantify metformin in a solution contaminated with terephthalic acid.
Accepted Answer
High-Performance Liquid Chromatography (HPLC) with a suitable UV-Vis detector is a commonly used and effective method for quantifying metformin in a solution contaminated with terephthalic acid. Here's why:
Selectivity: HPLC can separate metformin and terephthalic acid based on their different polarities and interactions with the stationary phase. This allows for accurate quantification of metformin without interference from the contaminant.
Sensitivity: UV-Vis detectors are sensitive to the absorbance of metformin in the UV region, enabling accurate quantification even at low concentrations.
Procedure:
1. Sample Preparation: Prepare a standard solution of metformin and a sample of the contaminated solution. Filter both solutions to remove any particulate matter.
2. HPLC Analysis: Inject known volumes of the standard and sample solutions into the HPLC system. Use a reversed-phase column with a suitable mobile phase (e.g., a mixture of water and acetonitrile) to separate metformin and terephthalic acid.
3. Quantification: Detect the eluting compounds using a UV-Vis detector set at the optimal wavelength for metformin. Quantify metformin by comparing its peak area to the standard solution.
Note: The specific parameters (e.g., column type, mobile phase composition, wavelength) will depend on the specific experimental conditions and the instrument used.
Selectivity: HPLC can separate metformin and terephthalic acid based on their different polarities and interactions with the stationary phase. This allows for accurate quantification of metformin without interference from the contaminant.
Sensitivity: UV-Vis detectors are sensitive to the absorbance of metformin in the UV region, enabling accurate quantification even at low concentrations.
Procedure:
1. Sample Preparation: Prepare a standard solution of metformin and a sample of the contaminated solution. Filter both solutions to remove any particulate matter.
2. HPLC Analysis: Inject known volumes of the standard and sample solutions into the HPLC system. Use a reversed-phase column with a suitable mobile phase (e.g., a mixture of water and acetonitrile) to separate metformin and terephthalic acid.
3. Quantification: Detect the eluting compounds using a UV-Vis detector set at the optimal wavelength for metformin. Quantify metformin by comparing its peak area to the standard solution.
Note: The specific parameters (e.g., column type, mobile phase composition, wavelength) will depend on the specific experimental conditions and the instrument used.
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