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What are the advantage of HPLC over traditional Liquid chromatographic method? (short question)
Accepted Answer
HPLC, or high-performance liquid chromatography, offers several advantages over traditional liquid chromatography methods:
* Higher resolution: HPLC employs smaller particles and higher pressure, resulting in better separation of compounds.
* Faster analysis: HPLC's high pressure allows for faster flow rates, reducing analysis time.
* Increased sensitivity: HPLC's advanced detectors, such as UV-Vis and fluorescence detectors, provide higher sensitivity, enabling detection of lower concentrations of analytes.
* Versatility: HPLC can be used to analyze a wide range of compounds, including non-polar and polar substances, making it suitable for various applications.
* Automation: HPLC systems are often automated, allowing for unattended operation and increased efficiency.
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