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Frequently Asked Question
Questions asked by users might not always be phrased in the clearest way.
What are the best ways to identify contaminations in HPLC analysis
Accepted Answer
Here are some ways to identify contaminations in HPLC analysis:
Peak Shape Analysis: Look for unusual peak shapes like tailing, fronting, or double peaks. These can indicate impurities.
Retention Time Shifts: Changes in retention times compared to known standards might signal contamination.
UV Spectrum Analysis: Analyze the UV spectra of peaks to look for absorption patterns inconsistent with the expected analyte.
Mass Spectrometry: If available, use MS to identify unknown compounds, including potential contaminants.
Standard Additions: Add known amounts of the analyte to the sample to see if the peak area changes proportionally. This can help differentiate between contamination and matrix effects.
Blank Analysis: Analyze a blank sample (without the analyte) to identify any peaks that might be from contaminants.
Method Validation: Validate your HPLC method to ensure accuracy and precision, which helps detect inconsistencies that may arise from contamination.
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