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What are the method used to detect colourless spots in TLC
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To detect colourless spots on a TLC plate, various methods can be employed. One common approach is to use UV light, as many compounds absorb UV radiation and appear as dark spots against the fluorescent background of the silica gel. Another method is to treat the TLC plate with iodine vapour, which forms a brown complex with many organic compounds, making them visible. Additionally, ninhydrin reagent can be used to detect amino acids, which react with ninhydrin to produce a purple colour.
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