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Ultraviolet and visible (UV-Vis) spectroscopy is widely used in chemistry to identify and quantify substances. It works by shining UV or visible light on a sample and analyzing the light that passes through. Different substances absorb light at different wavelengths, providing a unique 'fingerprint' for identification. Here are some specific uses:
Quantitative analysis: Measuring the amount of a substance by its absorbance. This is useful for determining concentrations in solutions or analyzing mixtures.
Qualitative analysis: Identifying unknown substances by their characteristic absorption spectra.
Study of chemical reactions: Monitoring changes in absorbance over time to follow reaction rates and study reaction mechanisms.
Analyzing materials: Studying the composition and structure of materials, like polymers or biological samples.