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What is the advantage of using gradient elution in HPLC ? (short answer)
Accepted Answer
Gradient elution has two main advantages in HPLC:
1. Improved resolution: By gradually increasing the strength of the eluent, the elution of different components is spread out over a longer period of time, leading to better separation and improved resolution.
2. Shorter analysis time: By starting with a weak eluent and gradually increasing its strength, it is possible to elute the desired components more quickly than isocratic elution, resulting in a shorter analysis time.
1. Improved resolution: By gradually increasing the strength of the eluent, the elution of different components is spread out over a longer period of time, leading to better separation and improved resolution.
2. Shorter analysis time: By starting with a weak eluent and gradually increasing its strength, it is possible to elute the desired components more quickly than isocratic elution, resulting in a shorter analysis time.
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