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What is the derivatization required in HPLC

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Derivatization in HPLC is a chemical process that modifies the analyte molecules to make them more suitable for HPLC analysis. This can be done to improve the analyte's chromatographic properties, such as its retention time, peak shape, or detectability. Derivatization can also be used to convert the analyte into a form that is more easily detected by the detector used in the HPLC system.

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