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What is the optimal way to perform an acid hydrolysis of proteins?
Accepted Answer
Acid hydrolysis of proteins is typically performed using strong acids like hydrochloric acid (HCl) or sulfuric acid (H2SO4) at elevated temperatures (around 100-120 °C) for several hours. This breaks the peptide bonds in the protein, yielding a mixture of free amino acids. The optimal conditions depend on the specific protein and the desired outcome. Factors to consider include the concentration of the acid, the reaction time, and the temperature. For complete hydrolysis, high acid concentrations and prolonged reaction times are usually required.
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