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Atomic Absorption Spectroscopy (AAS) is a quantitative analytical technique used to determine the concentration of a specific element in a sample. Here's a basic outline of the method:
1. Sample Preparation: The sample needs to be in a liquid or gaseous form. This often involves dissolving the solid sample in an appropriate solvent.
2. Atomization: The sample is introduced into a high-temperature environment (usually a flame or a graphite furnace) to vaporize the sample and produce free atoms of the analyte.
3. Light Source: A hollow cathode lamp emitting light specific to the element being analyzed is shone through the atomized sample.
4. Absorption: Atoms of the analyte in the sample absorb some of the light emitted by the hollow cathode lamp at specific wavelengths. This absorption is proportional to the concentration of the analyte.
5. Detection: The amount of light that passes through the sample is measured by a photomultiplier tube. The difference between the incident and transmitted light intensity is directly proportional to the concentration of the analyte.
6. Calibration: A calibration curve is created by measuring the absorbance of solutions with known concentrations of the analyte. This curve is used to determine the concentration of the analyte in the unknown sample.