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Frequently Asked Question
Questions asked by users might not always be phrased in the clearest way.
Advantage and disadvantage of normal phase and reverse phase
Accepted Answer
Normal Phase Chromatography (NPC)
Advantages:
* Higher resolution for polar compounds
* Compatible with non-polar mobile phases
* Stationary phase is more stable and has a longer lifespan
Disadvantages:
* Lower resolving power for non-polar compounds
* The mobile phase is more likely to evaporate
* The stationary phase can be more expensive
Reversed Phase Chromatography (RPC)
Advantages:
* Higher resolution for non-polar compounds
* Compatible with aqueous mobile phases
* Faster analysis time
* Greater sample capacity
Disadvantages:
* Lower resolution for polar compounds
* The stationary phase is less stable and has a shorter lifespan
* The stationary phase can be more expensive
Ultimately, the choice between normal phase and reversed phase chromatography depends on the specific analytes of interest and the desired separation performance.
Advantages:
* Higher resolution for polar compounds
* Compatible with non-polar mobile phases
* Stationary phase is more stable and has a longer lifespan
Disadvantages:
* Lower resolving power for non-polar compounds
* The mobile phase is more likely to evaporate
* The stationary phase can be more expensive
Reversed Phase Chromatography (RPC)
Advantages:
* Higher resolution for non-polar compounds
* Compatible with aqueous mobile phases
* Faster analysis time
* Greater sample capacity
Disadvantages:
* Lower resolution for polar compounds
* The stationary phase is less stable and has a shorter lifespan
* The stationary phase can be more expensive
Ultimately, the choice between normal phase and reversed phase chromatography depends on the specific analytes of interest and the desired separation performance.
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