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Introducing an energy level between E1 and E2 would increase the number of possible energy states for electrons in that region. However, the population of energy level 2 would likely decrease. This is because electrons would now have the option of occupying the newly introduced energy level, reducing the number of electrons that would occupy E2. The exact impact on the population of E2 would depend on the energy difference between the new level and E2, as well as the temperature of the system.