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Introducing an energy level between E1 and E2 would create a new energy state for electrons to occupy. According to Boltzmann's distribution, the population of each energy level is proportional to the exponential of the negative energy divided by kT (where k is Boltzmann's constant and T is temperature). With the new energy level, the population of E2 would likely decrease because some electrons would occupy the newly introduced energy level, thereby reducing the population of E2. The exact effect on the population of E2 depends on the energy difference between the new energy level and E1 and E2, as well as the temperature.