Frequently Asked Question

Questions asked by users might not always be phrased in the clearest way.

The table below shows the amount of 0.531 m standard x added to 50.0 ml sample solution and the corresponding HPLC readings. Find the concentration of compound x in the sample. Neglect the change in volume due to the addition of the standard. vstd (ul) 0 100.0 200.0 300.0 400.0 1084 1844 2473 3266 4010 peak Area

Accepted Answer

To find the concentration of compound X in the sample, you can use the standard addition method. Here's how:
1. Plot the data: Plot the peak area (y-axis) against the volume of standard X added (x-axis).
2. Find the intercept: Extend the line of best fit to the y-axis. The intercept represents the peak area that would be observed if no standard was added.
3. Calculate the unknown concentration: The slope of the line represents the change in peak area per unit volume of standard added. Use the equation:
`Concentration of X in sample = (Intercept / Slope)
Concentration of standard`
This will give you the concentration of compound X in the original 50.0 mL sample.


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