Top topic this week
Back to FAQs
Related FAQs
Frequently Asked Question
Questions asked by users might not always be phrased in the clearest way.
The table below shows the amount of 0.531 m standard x added to 50.0 ml sample solution and the corresponding HPLC readings. Find the concentration of compound x in the sample. Neglect the change in volume due to the addition of the standard. vstd (ul) 0 100.0 200.0 300.0 400.0 1084 1844 2473 3266 4010 peak Area
Accepted Answer
To find the concentration of compound X in the sample, you can use the standard addition method. Here's how:
1. Plot the data: Plot the peak area (y-axis) against the volume of standard X added (x-axis).
2. Find the intercept: Extend the line of best fit to the y-axis. The intercept represents the peak area that would be observed if no standard was added.
3. Calculate the unknown concentration: The slope of the line represents the change in peak area per unit volume of standard added. Use the equation:
`Concentration of X in sample = (Intercept / Slope)
Concentration of standard`
This will give you the concentration of compound X in the original 50.0 mL sample.
Articles you might like