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The table below shows the amount of 0.531 m standard x added to 50.0 ml sample solution and the corresponding HPLC readings. Find the concentration of compound x in the sample. Neglect the change in volume due to the addition of the standard. vstd (ul) 0 100.0 200.0 300.0 400.0 1084 1844 2473 3266 4010 peak Area A) 3.21 x 104 M B) 3.21 x 10+ M C) 1.58 x 103 m d) 3.75 x 103 m
Accepted Answer
This looks like a standard addition problem! To find the concentration of compound X in the sample, you'd follow these steps:
1. Plot the data: Graph the peak area (y-axis) against the volume of standard added (x-axis). This should yield a straight line.
2. Determine the slope: Calculate the slope of the line. The slope represents the change in peak area per unit volume of standard added.
3. Calculate the original concentration: The intercept of the line on the y-axis (where the volume of standard is 0) represents the original peak area of the sample. Divide this intercept by the slope, and you'll get the concentration of compound X in the sample.
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