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Temperature programming in gas chromatography analysis involves altering the temperature of the gas chromatograph column as the analysis progresses. It is used to optimize the separation and detection of components in a sample. By gradually increasing the temperature, components with lower boiling points elute first, followed by those with higher boiling points. This technique allows for the separation of a complex mixture of compounds over a shorter time compared to isothermal operation and can provide valuable information about the composition of the sample.