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What is the advantage of using gradient elution in HPLC?
Accepted Answer
Gradient elution in HPLC provides several advantages compared to isocratic elution:
- Improved separation of complex mixtures: By gradually changing the solvent composition over time, gradient elution can separate compounds with similar polarity more effectively. This is because the different compounds will elute at different points in the gradient, based on their relative affinities for the stationary and mobile phases.
- Increased peak capacity: Gradient elution can increase the number of peaks that can be resolved in a single run. This is because the different compounds will be eluted at different times, rather than all eluting at once as they would in isocratic elution.
- Reduced run times: Gradient elution can often reduce the run time compared to isocratic elution. This is because the gradient can be optimized to elute the desired compounds in a shorter amount of time.
- Improved peak shape: Gradient elution can help to improve the peak shape of the eluting compounds. This is because the gradient can be optimized to minimize peak tailing and fronting.
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