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Questions asked by users might not always be phrased in the clearest way.
Sure, here is the procedure for the tests of saponins, terpenoids, anthraquinones, phenols, and steroids:
Saponins
Frothing Test:
1. To a small amount of the extract, add a few drops of water and shake vigorously.
2. The formation of persistent froth indicates the presence of saponins.
Terpenoids
Salkowski Test:
1. To a small amount of the extract, add a few drops of concentrated sulfuric acid and heat gently.
2. A reddish-brown color indicates the presence of terpenoids.
Anthraquinones
Borntrager's Test:
1. To a small amount of the extract, add a few drops of 10% sodium hydroxide solution.
2. A reddish-violet color indicates the presence of anthraquinones.
Phenols
Ferric Chloride Test:
1. To a small amount of the extract, add a few drops of 1% ferric chloride solution.
2. A green, blue, or violet color indicates the presence of phenols.
Steroids
Liebermann-Burchard Test:
1. To a small amount of the extract, add a few drops of acetic anhydride and a drop of concentrated sulfuric acid.
2. A green, blue, or violet color indicates the presence of steroids.