Frequently Asked Question

Questions asked by users might not always be phrased in the clearest way.

Explain the Test for; sapponins, Terpenoids, anthraquinones, Phenols, and steroids.

Accepted Answer
Test for:

1. Saponins:
- Froth test: Saponins produce a stable froth when shaken with water.
- Hemolysis test: Saponins lyse red blood cells.

2. Terpenoids:
- Liebermann-Burchard test: Terpenoids give a green to blue color with acetic anhydride and concentrated sulfuric acid.
- Salkowski test: Terpenoids give a red to violet color with concentrated sulfuric acid.

3. Anthraquinones:
- Borntrager's test: Anthraquinones give a red to violet color with concentrated sulfuric acid.
- Alkaline hydrolysis test: Anthraquinones yield anthraquinone derivatives upon alkaline hydrolysis.

4. Phenols:
- Ferric chloride test: Phenols give a blue to green color with ferric chloride solution.
- Liebermann's test: Phenols give a blue to green color with concentrated sulfuric acid and sodium nitrite.

5. Steroids:
- Lieberman-Burchard test: Steroids give a green to blue color with acetic anhydride and concentrated sulfuric acid.
- Salkowski test: Steroids give a red to violet color with concentrated sulfuric acid.

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