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How can you detect the Colourless sports in paper chromatograph and TLC
Accepted Answer
To detect colourless spots in paper chromatography and TLC, you can use various methods:
1. UV lamp: Many compounds absorb UV light and appear as dark spots on a TLC plate or paper chromatogram under a UV lamp.
2. Iodine vapours: Iodine vapours react with many organic compounds to form coloured complexes, making the spots visible.
3. Ninhydrin: Ninhydrin is a chemical reagent that reacts with amino acids to produce a purple colour, making the amino acid spots visible.
4. Dragendorff's reagent: Dragendorff's reagent is used to detect the presence of alkaloids, which appear as orange or red spots.
5. Potassium permanganate: Potassium permanganate is a strong oxidising agent that reacts with many organic compounds to produce brown or purple spots.
6. Liebermann-Burchard reagent: Liebermann-Burchard reagent is used to detect the presence of steroids, which appear as green or blue spots.
7. Fluorescein: Fluorescein is a fluorescent dye that can be used to visualize colourless compounds under UV light.
8. Enzyme-linked immunosorbent assay (ELISA): ELISA is an immunological technique that can be used to detect the presence of specific proteins or other biomolecules in a sample.
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