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Sports created during paper chromatography or thin-layer chromatography (TLC) are often difficult to see because they are colorless. To detect these spots, several methods can be used. One common method is to use ultraviolet (UV) light. Many compounds absorb UV light, and when they do, they will fluoresce, making them visible. Another method is to use a chemical reagent that will react with the compound in the spot and produce a colored product. Finally, some compounds can be detected by their radioactivity if they are radioactive.