Top topic this week
Back to FAQs
Related FAQs
Frequently Asked Question
Questions asked by users might not always be phrased in the clearest way.
Difference between gradient elutionand isocratic elution
Accepted Answer
Gradient elution: In gradient elution, the composition of the mobile phase is gradually changed over the course of the separation. This can be done by changing the ratio of two solvents, or by adding a third solvent to the mobile phase. Gradient elution is often used to separate complex mixtures, as it can help to resolve peaks that would otherwise overlap.
Isocratic elution: In isocratic elution, the composition of the mobile phase is kept constant throughout the separation. This is typically done by using a single solvent, or by mixing two solvents in a fixed ratio. Isocratic elution is often used for simple separations, where the peaks are well-resolved.
Articles you might like