Top topic this week
Back to FAQs
Related FAQs
Frequently Asked Question
Questions asked by users might not always be phrased in the clearest way.
Difference between normal phase and reverse phase chromatography
Accepted Answer
In normal phase chromatography, the stationary phase is polar and the mobile phase is nonpolar. This means that polar compounds will interact more strongly with the stationary phase and will elute later than nonpolar compounds. In reverse phase chromatography, the stationary phase is nonpolar and the mobile phase is polar. This means that nonpolar compounds will interact more strongly with the stationary phase and will elute later than polar compounds.
Articles you might like