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When galactose is labeled with a 14c at C-3 (the aldehyde carbon) and passes through glycolysis, where will the label be detected?
Accepted Answer
The 14C label on C-3 of galactose will be detected in pyruvate, the end product of glycolysis. Here's why:
1. Galactose Metabolism: Galactose enters glycolysis after being converted to glucose-6-phosphate. This conversion involves isomerization steps that don't affect the position of the 14C label.
2. Glycolysis: During glycolysis, the carbon backbone of glucose is broken down into two molecules of pyruvate. The original C-3 of glucose (and therefore the labeled C-3 of galactose) will end up in the carboxyl group of pyruvate.
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