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When galactose is labeled with a 14c at C-3 (the aldehyde carbon) and passes through glycolysis, where would the label be detected?
Accepted Answer
The 14C label would be detected at the C-3 position of pyruvate. Here's why:
Galactose Metabolism: Galactose enters glycolysis after being converted to glucose-6-phosphate. The C-3 of galactose corresponds to the C-3 of glucose.
Glycolysis: During glycolysis, the C-3 carbon of glucose remains intact and does not participate in any reactions that would change its position.
Pyruvate Formation: The final product of glycolysis is pyruvate, which contains the original C-3 carbon from glucose.
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